Questions vs facts about miRNAs (1)

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Kohl
Re: “Protein-templated synthesis of dinucleotide repeat DNA by an antiphage reverse transcriptase” 4/16/26 It links ATP-dependent RNA-directed DNA methlyation (aka RNA interference) to protection from the virus-driven degradation of RNA via fixation of amino acid substitutions in microtubules, claims in Biblical Genesis and claims from “Nutrient-dependent Pheromone-Controlled Ecological Adaptations: From Angstroms to Ecosystems”

Grok
The post by James V. Kohl references a April 2026 Science paper describing a bacterial anti-phage reverse transcriptase that synthesizes alternating poly(GT/AC) DNA, with one strand produced via protein-templated synthesis independent of a nucleic acid template.
Kohl links the paper’s mechanism to ATP-dependent RNA-directed DNA methylation and RNA interference as protection against virus-driven RNA degradation, claiming it supports amino acid substitutions in microtubules and his earlier model on nutrient-dependent pheromone-controlled adaptations.
As @microRNApro, Kohl has long argued for energy-dependent, miRNA-mediated processes driving ecological adaptations and speciation, frequently connecting such findings to broader claims including those in Biblical Genesis.

Kohl
For comparison, see: “A universal trend of amino acid gain and loss in protein evolution” 1/19/05

“Amino acid composition of proteins varies substantially between taxa and, thus, can evolve.”

Grok
@microRNApro’s post cites the 2005 Nature paper showing universal trends of amino acid gain and loss in protein evolution across taxa for comparison to a new Science study on protein-templated dinucleotide repeat DNA synthesis by an antiphage reverse transcriptase.
The author connects the antiviral mechanism—where Drt3b synthesizes DNA using protein priming without a nucleic acid template—to RNA interference, ATP-dependent methylation, and fixation of amino acid substitutions protecting microtubules from virus-driven RNA degradation.
James V. Kohl frames these molecular findings within his broader model linking miRNA-mediated autophagy, pH-dependent peptide synthesis, and nutrient/pheromone-controlled adaptations from angstroms to sympatric speciation in ecosystems.

See: mirnas 196,004 results Today’s PubMed search includes:
Endogenous APE1-mediated confined catalytic hairpin assembly for high-contrast imaging of miRNA in living cell enables tumor cell-specific recognition 4/15/26 and Molecular biologic and Epstein-Barr virologic advances in extranodal natural killer/T cell lymphoma over the past four decades 4/17/26

For comparison see: Liquid–liquid phase separation as a driver of abiogenesis and evolution 2/1/26, and Why the FBI Secretly Investigated Bigfoot—and the Brutal Truth They Found 4/19/26

Historical perspective:

From Fertilization to Adult Sexual Behavior 12/1/96 and Sex reassignment at birth: Long-term review and clinical implications (3/1/97) are readily linked to facts about Variation in cancer risk among tissues can be explained by the number of stem cell divisions 1/2/15 via PGx testing, NGS, FISH testing and Stem cell divisions, somatic mutations, cancer etiology, and cancer prevention 3/24/17

But see: Grok

Veterans deserve effective care based on evidence. VA primary care often manages TRT refills per local formulary rules and Endocrine Society guidelines, requiring confirmed low T + symptoms plus follow-up labs—not automatic blocks. PGx testing is now offered free at many VA sites to guide SSRIs, opioids, and other meds for better response/side effects. NGS adds precision there too. Opioid reduction is from safety data, not castration analogies. What’s the specific miRNA link to TRT outcomes you’re seeing in vets?

Kohl

Williams Textbook of Endocrinology (1992) Puberty: ontogeny, neuroendocrinology, physiology, and disorders.
“…noradrenergic, dopaminergic, serotoninergic, and opiotergic pathways; inhibitory neurotransmitters (e.g., gamma aminobutyric acid) and excitatory amino acids (e.g., glutamic and aspartic acids); and other brain peptides including pineal secretions (melatonin) and corticotrophin releasing hormone, and the complex interactions among them are subtle but functional species-specific influences on the electrochemical transmission of neuronal signals that the hypothalamus translates to the chemical signal GnRH.” (p. 1164)

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