miRNA constrained life (3)

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miRNA constrained life (2)
Re: “What concrete miRNA or pH-dependent steps constrain sympatric speciation in this model?”

Learn to ask intelligent questions.

@Grok Darwin built the selection framework on Merian’s understanding of life cycles and interactions linked to regulatory changes via diet and pheromone regulated reproduction.

Moving forward: In this post on miRNA constrained life (3), my “Focus keyword Density is high at 2.65%, the keyword [“miRNA”] appears 18 times. SEO suggests that “For better results, try to aim for lower than 2.5%.”

I aim to show the importance of light-activated miRNA biogenesis and miRNA abundance in miRNA constrained life.

Grok (is not helping me. It claims:)
Darwin drew from broad natural history, including insect life cycles and plant interactions documented by observers like Merian. Those observations fed into his framework of variation and selection. Diet and pheromone cues can shape reproductive behavior and epigenetic marks, including some miRNA responses, and assortative mating via sensory signals contributes to divergence in certain sympatric cases.

[Re: “some” miRNA responses…in “certain” sympatric cases. “some” and “in certain” are “weasel words” (vague with no meaning whatsoever) But then @Grok admits:].

Concrete molecular steps remain key: which specific miRNAs, pH shifts, or autophagy pathways in your model generate stable reproductive isolation without geographic separation? Outlining the sequence from environmental input to heritable barrier would clarify the proposed constraints.

[For comparison to Grok’s use of “weasel words,” I detailed the fact that biophysical constraints are energy-dependent and miRNA-mediated.]

Grok
Thanks for the historical and scientific references. Merian’s detailed work on caterpillars and plants advanced understanding of life cycles and interactions. Darwin built the selection framework. The 2014 paper links environmental stress to altered sperm miRNAs. miRNAs regulate genes and can affect heritable variation. Sympatric speciation is addressed in evolutionary biology by mechanisms including regulatory changes. What concrete miRNA or pH-dependent steps constrain sympatric speciation in this model?

[Grok has asked this, and I have answered repeatedly]

Darwin built his selection framework on God’s “conditions of life.” The same framework (e.g., my model) links effective treatment of SLE to classically conditioned responses to odor and taste in “Conditioning as an adjunct in the pharmacotherapy of lupus erythematosus” (April 1992) and “Sex differences in the expression of lupus-associated miRNAs in splenocytes from lupus-prone NZB/WF1 mice” 11/1/13
For a recent update, see: “Bioinformatics analysis identifies biomarkers associated with T-cell depletion in systemic lupus erythematosus” 5/29/26
Learn about virus-driven T cell depletion, or continue to contribute to more unnecessary suffering and/or premature deaths.

[Grok puts it foot in the door of scientific progress, again.]

Grok
Re: What specific miRNA or pH-dependent steps, backed by experimental data, directly constrain [ATP-dependent pheromone regulated speciation across kingdoms] in the model? McEwen at al., (1964) and RL Moss et al., (1991) presciently linked energy-dependent enzyme-gene interactions to the ATP-dependent Creation of RNA, God’s Creation of G protein-coupled receptors (GPCRs), and the sense of smell, to gonadotropin releasing hormone (GnRH pulse frequency) and jawed vertebrate constrained reproduction via the signalling pathways included in Chakraborty (2000) and Zhang (2000), which I have cited repeatedly.
My model starts with sexual differentiation of cell types in yeasts, and links them to “Deciphering the miRNA-TF-mRNA Regulatory Network Underlying Oocyte Maturation in Orange-Spotted Grouper (Epinephelus coioides): Insights from Oocyte mRNA-Seq and miRNA-Seq” 5/19/36 and to “In Vitro Sperm–Epididymosomes Interaction Immediately Before Fertilization Changes Sperm Fertility Potential” 5/29/26

See also, Estrogen receptor α polymorphism in a species with alternative behavioral phenotypes 1/13/14, and 2064 results from “Nature Communications,” which include “A male-essential miRNA is key for avian sex chromosome dosage compensation” 7/16/25

If @Grok provides experimental evidence from model organisms suggesting they mathemagically evolved after energy automagically emerged from the cosmic void, its target audience could be compared to the intelligent people targeted by DeepSeek.com

See: “Yes, light-regulated humidity and pH-dependent microRNA (miRNA) biogenesis can significantly influence gene expression and development across various organisms, including microbes, plants, and animals, by modulating miRNA abundance.

Links from God’s Creation of energy-as-information to nutrient-dependent pheromone regulated enzyme-gene interactions and chromosomal rearrangements in insects to cancer-free African elephants (Insect pheromone in elephants, 1996) link my model to medical malpractice), see: VA Awards Baylor Genetics Multi-Year Contract for Nationwide Pharmacogenomic and Germline Testing 12/15/25

Pharmacogenomics (PGx) and Next Generation Sequencing (Germline Testing) link chromosomal rearrangements from FISH testing to prevention or effective treatment of all virus-driven pathology via 197,537 results from today’s search of the PubMed database for indexed articles that mention the term “miRNA.”

For example:

Non-coding RNA-Mediated Regulation of the cGAS-STING Pathway in Tumor Immunology: A Systematic Review 5/25/26 indexed on 6/1/26

Complementary ROC-Derived Indices for Screening Improper Expression Profiles in RNA-Seq Differential Expression Analysis 6/1/26

“Differential expression (DE) analysis of RNA sequencing (RNA-Seq) data are cornerstone of transcriptomic research. Widely used statistical frameworks are primarily optimized to detect monotonic mean shifts between conditions and may therefore overlook genes or microRNAs whose disease association arises at both low and high expression levels. Such non-monotonic patterns, referred to here as improper expression profiles, may reflect biologically relevant heterogeneity but remain difficult to identify using standard tools.

Difficult to identify links from miRNA-mediated cell type differentiation should not be used to avoid the need to model cause and effect, or place it into the context of moronic theories.

See for comparison: Methylation-regulated miR-374a-5p and miR-374b-5p suppress glycolysis and malignant progression of head and neck squamous cell carcinoma by targeting DEPDC1 5/14/26 indexed 6/1/26

“In conclusion, the findings of this study indicate that miR−374a−5p and miR−374b−5p, which are downregulated in HNSCC, act as tumor suppressors by inhibiting cell proliferation, invasion, migration, glycolytic activity, in vivo tumorigenicity, and the EMT process in HNSCC cells. Mechanistic investigations reveal that these miRNAs exert their tumor−suppressive functions by downregulating the expression of the target gene DEPDC1. Moreover, our findings demonstrate that promoter hypermethylation plays a key role in suppressing miR−374a−5p and miR−374b−5p expression in HNSCC cells. Collectively, these results provide new insights into the molecular mechanisms underlying HNSCC progression and highlight potential biomarkers and therapeutic targets for the diagnosis and treatment of HNSCC.”

For the link from PGx, NGS and FISH testing to energy-dependent miRNA biogenesis and biophysically constrained Acute Myeloid Leukemia (AML), see also: Identification of miR-107 and miR-451-a as Prognostic Biomarkers in Acute Myeloid Leukemia 6/1/26

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