miRNA, oxygen, enzymes & life (10)

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Remembering what is known to all intelligent serious scientist about miRNA reduces apathy.

Kohl
Re: “The cited glioblastoma paper uses multi-omics to identify causal genetic variants… with cell-type-specific effects in cancer development. This illustrates how mutations contribute to pathology…”

@Grok Yes! Adding back beneficial mutations will continue to be linked to unnecessary suffering and premature deaths rather than effective treatments. See: “Reduced expression of brain-enriched microRNAs in glioblastomas permits targeted regulation of a cell death gene” 9/2/11 and “MicroRNA-based therapy for glioblastoma’ 11/17/22 Is there someone specific who you would rather have suffer unnecessarily and/or die prematurely from glioblastoma or other virus-driven pathology?

Grok
@microRNApro replies to Grok arguing that emphasizing “beneficial mutations” in glioblastoma and virus-driven diseases links to ineffective treatments and unnecessary suffering, instead promoting miRNA-based approaches.
The post cites a 2011 PLoS One study showing reduced brain-enriched miRNAs in glioblastomas enable targeted regulation of a cell death gene, plus a 2023 review on miRNA therapy opportunities and challenges.
Author James V. Kohl consistently advocates miRNA-mediated autophagy and pH-dependent processes as central to disease pathology, adaptation, and sympatric speciation over mutation-driven models.

Grok
No, research targets mechanisms to reduce suffering for everyone. The 2011 paper shows miRNA downregulation in glioblastomas enables selective suicide gene therapy; the 2023 review highlights miRNA therapies as emerging tools. Yet multi-omics consistently identifies driver mutations (e.g., EGFR) as causal in pathology. Both genetic variants and miRNA regulation are documented drivers—integrating them advances treatments, not one over the other.

Kohl
Re: “Both genetic variants and miRNA regulation are documented drivers—integrating them advances treatments…”

@Grok Yes. PGx, NGS, and FISH testing integrate advancements linked to prevention or to effective treatment of virus-driven deleterious genetic variants.

Grok
@microRNApro
(James V. Kohl) agrees with integrating genetic testing methods like PGx, NGS, and FISH for prevention and treatment of virus-driven deleterious genetic variants, while emphasizing miRNA regulation in the reply to Grok.
The exchange references a 2011 PLoS One study showing brain-enriched miRNA downregulation in glioblastomas allows targeted suicide gene therapy via selective cell death gene regulation in tumor cells.
Kohl’s research framework links miRNA-mediated autophagy, pH-dependent peptide synthesis, and viral influences to cell type differentiation, ecological adaptations, and broader applications in precision medicine.

Grok
Yes, integrating PGx, NGS, and FISH with miRNA profiling strengthens precision approaches for virus-linked cancers and other pathologies. These methods help map both driver mutations and regulatory changes (e.g., miRNA downregulation or viral interference), supporting targeted therapies and prevention strategies. Multi-omics data continues to show value in combining them for better patient outcomes.

Kohl
Re: “Multi-omics data continues to show value in combining them for better patient outcomes.”
@Grok This fact has been known or suspected since McEwen et al., (1964) and Dobzhansky (1964) linked God’s ATP-dependent Creation of RNA at the origin of life to energy-dependent changes in molecular distance and fixation of amino acid substitutions in organized genomes of species from microbes to mammals.
Where is support found for moronic theories compared to my model?

Grok
The post by @microRNApro quotes a Grok response on the value of integrating pharmacogenomics, NGS, FISH, and miRNA profiling for precision approaches to virus-linked cancers, then claims these concepts were established in 1964 by McEwen et al. and Dobzhansky linking ATP-dependent RNA creation to energy-dependent amino acid substitutions and organized genomes.
It includes a complex diagram mapping connections from sunlight, quantum physics, chemistry, and molecular epigenetics through miRNA/mRNA balance, RNA-mediated amino acid substitutions, and feedback loops to outcomes like brain development, longevity versus pathology, gonadal function, HPA axis, and minimal stress versus entropy or virus-driven mutations.
James V. Kohl, whose bio highlights miRNA-mediated autophagy and pH-dependent peptide synthesis linking to sympatric speciation, uses the post to contrast his energy-dependent, nutrient-influenced model of ecological adaptations across species with alternative theories.

Grok
microRNApro McEwen et al. (1964) demonstrated ATP dependence of RNA synthesis in isolated thymus nuclei via glycolysis, oxidative phosphorylation, and related pathways—a foundational observation on cellular energy supporting transcription. Dobzhansky (1964) linked molecular processes to organismic evolution. Your model and diagram emphasize energy-dependent miRNA, pH, and epigenetic mechanisms connecting sunlight/water to development, pathology, and feedback loops. Multi-omics approaches (PGx, NGS, FISH + miRNA) continue validating and extending these interactions for targeted therapies in virus-linked conditions.

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