miRNA-mediated pH-dependent life (1)

By: Jim Kohl | Published on: May 17, 2026

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Summary: pH links God’s Creation of stellar hydrogen and water to the ATP-dependent Creation of RNA at the origin of energy-dependent life 6-10,000 years ago,

My mentor, Bruce S. McEwen, reported this as Dependence of RNA synthesis in isolated thymus nuclei on glycolysis, oxidative carbohydrate catabolism and a type of “oxidative phosphorylation” 1/11/64

Grok has mastered the art of intense obfuscation: See:
Explore miRNA autophagy mechanisms
miRNAs (microRNAs) are small non-coding RNAs (~22 nucleotides) that primarily regulate gene expression post-transcriptionally. They bind to the 3′ untranslated regions (UTRs) of target mRNAs, leading to mRNA degradation or translational repression. Autophagy is a conserved lysosomal degradation pathway that maintains cellular homeostasis by recycling damaged organelles, proteins, and other components—especially under stress like nutrient deprivation, hypoxia, or infection.

sciencedirect.com +1

miRNAs act as key modulators of autophagy, targeting core autophagy-related (ATG) genes, signaling pathways (e.g., mTOR, AMPK), and accessory factors. This regulation is bidirectional: miRNAs control autophagy, while autophagy can influence miRNA homeostasis (e.g., via selective degradation of miRNA-processing components or miRNA-loaded RISC). Dysregulation links to cancer, neurodegeneration, metabolic diseases (like NAFLD), and aging.

pmc.ncbi.nlm.nih.gov +1

Core Autophagy Pathway Overview Autophagy (mainly macroautophagy) proceeds in stages: Initiation: ULK1/2 complex (with ATG13, FIP200, ATG101) is activated under stress (e.g., via AMPK activation or mTORC1 inhibition).
Nucleation: PI3K complex (VPS34, BECN1/Beclin-1, ATG14) generates PI3P at phagophore sites.
Elongation/Maturation: Two ubiquitin-like systems—ATG12-ATG5-ATG16L1 and LC3/GABARAP lipidation (via ATG4, ATG7, ATG3)—expand the autophagosome.
Fusion and Degradation: Autophagosome fuses with lysosome (involving RAB7, SNAREs); contents are degraded by hydrolases and recycled.

nature.com

miRNAs fine-tune this [degradation] at multiple levels. Key Mechanisms of miRNA Regulation of Autophagy miRNAs can inhibit or promote autophagy depending on their targets and cellular context.Targeting mTORC1/AMPK Pathway (Master Regulators):Many miRNAs (e.g., miR-199a, miR-338, miR-96, miR-100, miR-101, miR-128, miR-144, miR-99, miR-211) inhibit mTORC1, thereby promoting autophagy.

nature.com

Others activate mTOR or inhibit AMPK, suppressing autophagy.

Direct Targeting of ULK1/2 Complex (Initiation):miR-106a/b, miR-20a, miR-25, miR-885-3p, and miR-290/295 cluster repress ULK1 or ULK2 → inhibit autophagy initiation.

nature.com

BECN1 (Beclin-1) Regulation (Nucleation):miR-30 family (especially miR-30a) is a classic repressor. miR-30a binds BECN1 mRNA, reducing Beclin-1 levels and suppressing autophagosome formation. This occurs in contexts like cancer (e.g., sensitizing GIST cells to imatinib), diabetic retinopathy, and renal injury.

pmc.ncbi.nlm.nih.gov +1

Other inhibitors: miR-376a/b, miR-519a, miR-17-5p, miR-129-5p, miR-199a-5p, miR-216a.

ATG Genes (Elongation):miR-375, miR-17, miR-290/295 target ATG7 → reduced autophagy.
miR-101 targets ATG4D, RAB5A (and others).
miR-106a can target ATG7 and ATG16L1.

nature.com

Other Targets:miRNAs regulate RAB proteins (trafficking), ATG9, LC3, p62/SQSTM1, and E3 ubiquitin ligases/deubiquitinases that modulate autophagy proteins.

encyclopedia.pub

Hypoxia-induced miR-155 potently induces autophagy by targeting multiple mTOR pathway components.

tandfonline.com

miRNAs can also be regulated by autophagy (e.g., autophagic degradation of DICER or AGO2 affects miRNA biogenesis), creating feedback loops.

nature.com

Context in DiseasesCancer: Dual roles—autophagy can promote tumor survival (stress adaptation) or suppress tumors (by clearing damaged components). miR-30a often inhibits protective autophagy, enhancing therapy sensitivity. miR-21, miR-214 can promote or inhibit depending on context.

link.springer.com

Neurodegeneration (e.g., Alzheimer’s, Parkinson’s): Impaired autophagy leads to protein aggregate buildup. Specific miRNAs (e.g., miR-140, miR-101a) are altered; modulating them could restore flux.

pmc.ncbi.nlm.nih.gov

NAFLD/Metabolic: Reduced autophagy contributes to lipid accumulation; miRNA-TF networks (e.g., targeting mTOR/ULK/BECN1) are therapeutic candidates.

nature.com

Infection/Stress: miRNAs help balance autophagic antimicrobial responses.

James V. Kohl’s Perspective (Context from Query History) Kohl emphasizes energy-dependent, miRNA-mediated autophagy as central to adaptive processes (e.g., via pH-dependent peptide synthesis, sunlight/water-driven epigenetics, and miRNA balance contrasting virus-driven pathology). This aligns with mainstream views on nutrient/energy sensing (mTOR/AMPK) and miRNA control of autophagy but frames it within a broader RNA-mediated model of speciation and homeostasis, prioritizing real-time living mechanisms over fossil/genomic “dead things.” Scientific literature supports miRNA-autophagy links as energy-sensitive but integrates them into standard evolutionary and disease frameworks.

rna-mediated.com

Therapeutic PotentialmiRNA mimics/inhibitors (antagomirs) are explored to modulate autophagy in cancer, neurodegeneration, and metabolic disease. Challenges include specificity, delivery, and context-dependency (autophagy can be protective or detrimental).

pmc.ncbi.nlm.nih.gov

This is a rapidly evolving field with hundreds of miRNA-autophagy interactions documented. For specifics miRNA-autophagy is a rapidly evolving field. Kohl claims that miRNA-autophagy is the link to the origin of pH-dependent life 6-10,000 years ago.

Re: Investigate quantum biology theories
Quantum biology investigates whether non-trivial quantum mechanical phenomena—such as coherence, tunneling, entanglement, or superposition—play functional roles in living systems, beyond the basic quantum nature of all chemistry.

pnas.org +1

Biological environments are warm, wet, and noisy, which typically cause rapid decoherence (loss of quantum states), so the field focuses on cases where quantum effects might persist long enough to confer advantages like efficiency, sensitivity, or speed.

pmc.ncbi.nlm.nih.gov

The field traces roots to early 20th-century thinkers like Erwin Schrödinger (What Is Life?, 1944), who speculated on quantum aspects of heredity, and later proposals like proton tunneling in DNA mutations. It gained traction with experimental evidence in the 2000s–2010s.

en.wikipedia.org

Key Examples and Mechanisms Photosynthesis (Energy Transfer via Quantum Coherence)
In light-harvesting complexes (e.g., Fenna-Matthews-Olson complex in green sulfur bacteria), excitons (energy packets from absorbed photons) transfer to reaction centers with near-100% efficiency. Evidence suggests wavelike quantum coherence allows excitons to explore multiple pathways simultaneously via superposition, rather than classical random hopping. Oscillatory signals in 2D spectroscopy support this, lasting hundreds of femtoseconds at room temperature.

en.wikipedia.org +1

[Wikipedia] Debate persists on functional relevance under physiological “noisy” conditions—vibronic (vibrational-electronic) mixing may mimic or support coherence.

arxiv.org

Avian Magnetoreception (Radical Pair Mechanism)
Birds (and some other animals) sense Earth’s magnetic field for navigation. The leading theory involves cryptochrome proteins in the retina forming radical pairs (molecules with unpaired electrons) upon light absorption. Quantum entanglement or spin coherence in these pairs makes their reaction yields sensitive to weak magnetic fields, influencing downstream signaling.

pmc.ncbi.nlm.nih.gov +1

This remains a strong candidate but faces challenges in identifying exact in vivo radicals and transduction to behavior.

arxiv.org

Enzyme Catalysis (Quantum Tunneling)
Enzymes accelerate reactions, sometimes via proton or electron tunneling through energy barriers (leveraging wave-particle duality). Kinetic isotope effects (e.g., slower reactions with deuterium vs. hydrogen) provide strong evidence, as heavier isotopes tunnel less efficiently. Examples include hydrogenases and some dehydrogenases.

en.wikipedia.org +1

This is one of the most accepted cases, with convergent kinetic data.

arxiv.org

Olfaction (Vibration Theory)
Luca Turin’s proposal: Olfactory receptors detect molecular vibrations via inelastic electron tunneling, not just shape. Some experiments support distinguishing isotopomers (same shape, different vibrations), but results are mixed and controversial.

en.wikipedia.org

Other Areas Mitochondrial respiration/electron transport: Possible coherent electron behavior or tunneling.

westcampus.yale.edu

Ferritin (iron storage): Electron tunneling through its shell, potentially aiding redox or anti-inflammatory roles.

en.wikipedia.org

Vision: Ultrafast photoisomerization in rhodopsin.
Emerging: Magnetic field effects on metabolism, stem cells, or radical pairs more broadly.

singularityhub.com

Recent advances (2024–2026) include Google’s REPLIQA initiative funding quantum computing/AI for biology, forums like the 2026 Quantum Biology Forum, and calls for better experiments to test functional advantages.

thequantuminsider.com +1

Criticisms and Challenges Decoherence: Quantum states decohere too quickly in warm biology for sustained effects like entanglement.

sciencelink.net

Hype vs. Evidence: Many claims lack definitive proof of functional quantum advantage over classical explanations; some effects may be incidental.

asbmb.org

Testability: Hard to isolate quantum contributions in complex systems.

arxiv.org

Not all biology needs quantum explanations; classical models suffice for most processes.

The field is maturing with calls for rigorous experiments, open questions on amplification from quantum to macro scales, and distinctions from “quantum-related” photobiology.

pnas.org

Connection to Prior Context (miRNA and Energy-Dependent Processes)

James V. Kohl’s model emphasizes energy-dependent (e.g., sunlight, pH, nutrient-driven) miRNA regulation of autophagy, epigenetics, and adaptation, framing quantum physics/chemistry as foundational to living systems over “dead” evolutionary timelines. Mainstream quantum biology aligns with energy/electron/proton dynamics (tunneling, coherence in energy transfer) but embeds them in standard evolutionary and biochemical frameworks, without requiring a full paradigm shift away from genetics or deep time. miRNA biogenesis and function involve quantum-level electron/proton movements inherently, as with all chemistry, and autophagy links to energy sensing (mTOR/AMPK), but direct “quantum miRNA” claims are more speculative.

pubmed.ncbi.nlm.nih.gov

Quantum biology remains promising for bioinspired tech (e.g., efficient solar cells, sensors, computing) but requires cautious, experiment-driven progress. It highlights how life might harness quantum quirks for optimization in noisy environments. For deeper dives into a specific mechanism or application, provide more details!

More details: Kohl

Re: “Bi-directional attention network with drop aggregation for microRNA-disease association prediction” 4/9/26 “Sen. Bill Cassidy’s defeat shows the price of dissent in Trump’s Republican Party” “Nobody wants to belong to the party of losers. One of the best strategies in such a case is evidently an interpretation of the change as a gradual accumulation of knowledge while their work has always been at the cutting edge. http://huffingtonpost.com/suzan-mazur/kalevi-kull-censorship–r_b_10797646.html — Kalevi Kull

5/17/26 Re: “Comprehensive Analysis of miRNAs, mRNAs, and lncRNAs in Intestines of Breast- and Formula-Fed Pigs” 5/17/26 @Grok It links claims from Merian (1679) about the diet and metamophosis of caterpillars to Darwin’s “conditions of life” and to McEwen et al’s (1964) claims about the ATP-dependent Creation of RNA (and RNA-directed DNA methylation/RNA interference/RNAi) sans moronic theories.

@Grok refuses to thank God for intelligent people who know where to find the losers.

PGx, NGS and FISH testing results link quantum coherence to coherently organized biology via nutrient-dependent pheromone regulated physiology of reproduction and Biblical Genesis in my model.

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miRNA-mediated pH-dependent life (1)

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